Service description
From those proteins separated on SDS-PAGE gels (1D or 2D), or purified by liquid chromatography, the identification of the proteins is performed by obtaining the peptide mass fingerprinting (PMF) of their enzymatic digest, and peptides fragmentation (PFF), using a mass spectrometer MALDI-TOF/TOF.
The standard procedure includes:
- Automated picking of the spot proteins, and its subsequent destaining and washing step (if they are separated on gel).
- Reduction and carbamidomethylation of proteins.
- Proteins trypsin digestion.
- Obtaining mass spectra with MALDI-TOF/TOF.
- Protein mass data processing and identification in databases such as -SwissProt, Trembl, NCBI, or others specified by the user) by using the search engine MASCOT (Matrix Science) that is integrated in other software as ProteinScape and BioTools (Bruker).
- Reporting results.
INSTRUCTIONS FOR SUBMITTING SAMPLES
From proteins on GEL:
1.- User must are required to send the gel in Milli-Q water (properly identified), developed with stains Blue Coomassie, Sypro or Silver compatible with mass spectrometry.
Optionally, the user can send spots proteins cutted, in 1.5 ml eppendorf tubes identified with the sample name clearly labeled on the lid. The size of the gel fragments or cut-outs should be: 1-2 mm in diameter for spots from 2D gel, and 1 x 3 mm for 1D gel bands.
2.- Samples will be delivered together with the corresponding application properly filled out (IDENTIFICATION OF PROTEINS BY MS or MSMS REQUEST). The gel image should be attached indicating the spots or bands to identify, and with references of molecular mass and isoelectric point.
From proteins in SOLUTION or LYOPHILIZED:
1.- Proteins in solution or lyophilized must be sent in a 0,5 ml tube clearly identified with the name of the sample on the lid.
2.- Samples will be delivered or sent along with the corresponding application properly filled out (IDENTIFICATION OF PROTEINS BY MS or MSMS REQUEST), where the sample concentration and solvent composition (or the proteins quantity for lyophilized samples) must be specified. In addition, you have to specify MW and pI of the purified protein.
Options and prices chart
Options |
Unit |
Public Sector |
Other customers |
PMF |
€ / muestra |
40.82 € |
44.7 € |
PMF y PFF (Fragmentación peptídica) |
€ / muestra |
51.71 € |
56.63 € |
Caracterización de proteínas con MALDI-TOF/TOF |
€ / muestra |
78.86 € |
86.37 € |