Generation of stable transgenic zebrafish

Service description

This service includes the following steps: A/ Evaluation of vector* activity 1. Microinjection of the construct* into one-cell stage embryos. 2. Evaluation of vector activity in 24-48hpf embryos with a fluorescence scope. Delivery time: 2 weeks. Delivery format: The customer will receive photos from F0 embryos at 24-48hpf to evaluate the fluorescence activity of the provided vector. Approval by the customer is required in order to proceed with the following step. * The Functional Genomic Service does not provide cloning services. Contact the manager for advice on vector selection and/or cloning. The construct is to be provided by the customer. We require 20µl at a minimum concentration = 50ng/µl (from kit). Note: Without obvious technical issues, the price for step A (see below) will be charged independently of the final outcome. B/ Generation of a zebrafish or medaka stable transgenic line 1. Raising of F0 positive embryos to adulthood. 2. Screening of F0 adult fishes: - Outcross: individual F0 males and females are outcrossed with WT females and males respectively to identify Founders. - Selection of fluorescence positive F1 embryos from founder/s. Delivery time: approximately 6 months. Delivery format**: The customer will receive bleached F1 transgenic embryos. ** If required, transport is to be paid by the customer.