Numerical methods of cryo electron tomography.

The Castaño group is devoted to the advancement of the numerica methods for cryo electron tomography, including all aspects of the pipeline: alignment, reconstruction, particle location, subtomogram averaging and classification. This includes exploring protocols for cooperative use of further imaging techniques from Structural and Cellular biology (as superresolution light microscopy) towards the integration of information on multiple scales in space and time, aiming at the completion of mechanistic studies of key life processes. The group is commited to the production of robust, user-friendly software, integrating the newest advancements from numerical mathematics (from heuristic optimization to deep learning) as algorithms specifically tailored for cryo electron tomography, and designed for an optimal use of high-performance computing resources as GPUs or large scale clusters.