Optical microscopy

Service description

Observation of meteorite surfaces and electronic components surfaces using optical microscopy techniques. Operating in reflected and transmitted/polarized light. Petrografic microscope Zeiss Axio Scope in clean room environment ISO-8 (class 100,000).

Service description

The core-repository has an important collection of thin sections, cuttings from deep drilling, and other samples. The core-repository offers the service of digital microphotographs with a HDMI camara, either with a transmitted or reflected light optical microscope, a binocular or microphotographs of samples using dino-lite type devices.

Service description

Optical microscopy equipment: Widefield automated Epifluorescence Microscopy, Laser Scanning Confocal Microscopy, Spinning Disk confocal Microscopy, Two-photon Microscopy, Fluorescence Correlation Microscopy, TIRF, FLIM, Super-Resolution Microscopy and Image Analysis. VARIANTS: Zeiss780Confocal: Confocal and multiphoton Microscope associate to a motorized in xyz inverted AxioObserverZ1 stand; Confocal: laser 405, 458, 488, 514, 561, 594 and 633 controlled by AOTF; detection system based on one spectral GaAsP detector, 2 PMTs for fluorescence plus 1 for transmission; equipped with high numerical apertures optics and specialized optics for FCS/FCCs experiments; includes macro editor for complex experiments; Zen Black acquisition software; temperature and Co2 control. Available for self-service or with assistance. Zeiss780Multiphoton: on motorized in xyz inverted AxioObserverZ1 stand. Laser: MaiTai HP DS 2,1W 80MHz 690-1040 nm; AOM modulator for wavelength switching with GDD pulse control system; external GaAsP two-channel detector; equipped with specialized C-APO optics for infrared correction; includes macro editor for complex experiments; Zen Black acquisition software; temperature and Co2 control. Available for self-service or with assistance. AndorDragonfly: Super High-speed Spinning Disk Acquisition System, associated with fully motorized Nikon inverted microscope; motorized stage and objective piezo for z control; laser lines 405, 445, 488, 514, 561, 594 and 637. Three optical paths, for widefield, confocal y TIRF. Software with deconvolution module, SRRF and prepare for STORM. Temperature and CO2 control. Available for self-service o with assistance. ThunderImagingSystem: Widefield microscope associated with fully motorized DMI8000 stand with high-speed focusing system,; LED-based illumination with 6 channels between 365-650nm controlled by TTL; high sensitivity and efficiency sCMOS digital camera with high quantum efficiency; Sedat high-speed filters configuration control; equipped with high numerical aperture optics and low-medium magnification multi-immersion optics; LASX software with 3D, deconvolution and screening modules; out-of-focus light clearing system using real-time GPU algorithms; high performance workstation; with universal adapter and 96-well plate adapter; temperature and CO2 control. Available for self-service o with assistance. LeicaAF7000: Widefield microscope associated with motorized DMI6000 stand; LED-based illumination (Sola) from 405 to 650 nm; equipped with two cameras one for fluorescence (OrcaR2) and one true colour (DFC550); high-speed filter control; pinkel configuration; equipped with long working distance optics; controlled by a micromanager; with universal adapter and 96-well plate adapter; LASX software with screening modules. Available for self-service o with assistance. LeicaStellaris8FALCON. Laser Scanning Confocal associated with motorized in xyz inverted DMI8000 stand; lase lines 405 and pulsated white laser; spectral detection system (AOBS) with 3 HyDS and 2 HyDX detectors for fluorescence plus one for transmission; equipped with high numerical aperture optics and specialized optics for FCS/FCCS experiments; equipped with FALCON module for FLIM and FERT experiments based in fluorescence lifetime; LASX software with 3D, deconvolution and screening modules; with universal adapter and 96-well plate adapter; temperature and CO2 control. Available for self-service o with assistance. ImagingWorkstation1: Workstation equipped for advanced image processing, contains Huygens Pro Deconvolution software and free-access software (ImageJ/Fiji; Cell profiler; ilastik). Available for self-service o with assistance. ImagingWorkstation2: Workstation equipped with Volocity image analysis software and free-access software (ImageJ/Fiji; Cell profiler; ilastik). Available for self-service o with assistance