Isothermal Titration Calorimetry (ITC) | Consejo Superior de Investigaciones Científicas

Service data

Support unit: Servicio Interacciones Biofísicas: SPR e ITC
Institute: INSTITUTO DE QUIMICA MEDICA
Locality: Madrid (Madrid)
Service's web: http://www.iqm.csic.es/

Service's description

La unidad dispone de un equipo Biacore X-100 y de un Microcal PeaQ-ITC que permiten detectar y monitorizar interacciones entre dos o más moléculas en tiempo real sin emplear ningún tipo de marcaje. El tipo de interacciones estudiadas pueden ser: proteína-proteína, proteína-péptido, proteína-molécula pequeña, péptido-péptido y ADN-molécula pequeña. Los experimentos que pueden llevarse a cabo permiten determinar la especificidad entre dos o más ligandos y/o caracterizar la cinética y afinidad de dicha interacción. Con el análisis de los datos se obtienen las constantes de velocidad así como las de asociación/disociación del complejo formado (SPR). También se puede determinar la Kd, entalpía y entropía de la interacción mediante microcalorimetría (ITC).

Service description

ITC is a methodology based in the absorption or liberation of heat when an interaction between two molecules happens at constant temperature.With ITC it is possible to obtain the following parameters: Kb, enthalpy, entropy and stoichiometry of the interaction. The measures are in solution, which is perfect to complement the SPR unit. This methodology permits the use of differents solvents and it is not necessary to label the sample.

Options and prices chart
Options	Unit	Public Sector	Other customers
Tarifa Servicio ITC experimentos y analisis	euro / día	368.71 €	403.82 €

Service description

Isothermal Titration Calorimetry (ITC) is recognized as the gold standard technique to characterize molecular interactions. Major advantages of ITC include the facts that there is almost no restriction as to the type of molecules analysed including proteins, DNA or low molecular weight compounds, that the analysis does not involve ligand immobilization and that experiments can be conducted in virtually any buffer system and over a wide temperature range. ITC measures the heat released (exothermic reaction) or adsorbed (endothermic reaction) during a molecular interaction. This technique permits the determination of the complete set of thermodynamic binding parameters in a single experiment, such as the dissociation constant and changes in enthalpy, entropy and Gibbs free energy. Furthermore, information on the binding stoichiometry can be obtained. The Scientific Instrumentation service of the EEZ has a Nano ITC instrument that requires much less ligand amounts than more traditional instruments.

Options and prices chart
Options	Unit	Public Sector	Other customers
Recorrido titulación hecho por el técnico por hora	Curva de valoración	27.71 €	33.67 €

Service description

Isothermal Titration Calorimetry (ITC) Equilibrium, stoichiometry and thermodynamic parameters of both association and dissociation reactions are obtained by measuring the released absorbed heat. This service is comprised of one piece for low volume cells, allowing assessments to be done in a shorter time using small amounts of sample, and a second for standard volume cells to determine more accurately dissociation constant values. Differential Scanning Calorimetry (DSC) DSC or Differential Scanning Calorimetry serves to determine changes in heat capacity in a temperature range by measuring the heat absorbed or released by samples. The Nano-DSC is specifically designed for measuring thermal transitions in small volumes of diluted bio-molecule solutions while these are being heated or cooled. Transition enthalpies and temperatures (i.e. melting points) as well as changes in heat capacity can be obtained. This technique is particularly suited for the analysis of biomolecule thermal stability.

Options and prices chart
Options	Unit	Public Sector	Other customers
Servicio de ITC LV (low volumen)	€ / 5 horas	32.11 €	55.04 €
Servicio de ITC SV (Standard volumen) ó DSC	€ / 5 horas	16.81 €	28.82 €

Service description

Isothermal Titration Calorimetry (VP-ITC; GE HealthCare-Microcal) ITC is a thermodynamic technique that directly measures the heat released or absorbed during a biomolecular binding event (protein-small molecule, protein-protein, target-drug, enzyme-inhibitor, antibody-antigen, protein-DNA, protein-lipid, small molecule-small molecule). Measurement of this heat allows accurate determination of binding constants (KB), reaction stoichiometry (n), enthalpy (¿H) and entropy (¿S), thereby providing a complete thermodynamic profile of the molecular interaction in a single experiment.

Options and prices chart
Options	Unit	Public Sector	Other customers
General	euro / hora	10.01 €	15.01 €

Support unit	Support unit Information	Selected service	Other services
Servicio Interacciones Biofísicas: SPR e ITC IQM - Madrid
SERVICIO DE INSTRUMENTACIÓN CIENTÍFICA EEZ - Granada
Plataforma de Interacciones Biomoleculares (BIP) CICCARTUJA - Sevilla
PREPARACION Y CARACTERIZACION DE SOFT MATERIALS ICMAB - Cerdanyola del Vallès