PIF2024 - ANALISIS FUNCIONAL DE LA SEGREGACION CROMOSOMICA Y DE LA GAMETOGENESIS (PID2023-152857NB-I00)

The aim of the current project is to obtain fundamental knowledge on the processes controlling the reductional chromosome segregation process that take place during gametogenesis. We are particularly interested to study the interplay between the recombination machinery and the process of chromosome synapsis and maturation of crossovers (COs) in the natural occurring context of a proteinaceous structure that operates as a conserved scaffold complex, the synaptonemal complex (SC). The dysregulated control of this intricate process, wherein numerous self-inflicted double-strand breaks (DSBs) are repaired, leading to the generation of a limited number of crossovers (COs) during meiotic prophase I, is currently recognized as a significant contributor to mutagenesis in the germline of both human and mouse genome.Centered on these topics, the general objectives can be divided into three objectives:1-Elucidating Mechanisms Underliying CO Designation, Maturation and their Interplay with Chromosome Synapsis. This aim seeks to systematically investigate the conserved pathway governing meiotic dynamics, specifically by unraveling the intricate regulatory mechanisms associated with CO designation and maturation and their relationship with chromosome synapsis. Despite the established knowledge of these processes at a transient and descriptive level, a mechanistic comprehension remains notably incomplete.2-Function of H2AX phosphorylation in mouse meiosis.